期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 178, 期 1-2, 页码 46-50出版社
ELSEVIER
DOI: 10.1016/j.molbiopara.2011.03.001
关键词
Functional genomic tools; Schistosoma; Phenol oxidase; Tetraspanin; Argonaute; Superoxide dismutase
资金
- National Institute of Allergy and Infectious Diseases [AI065622, AI081107]
- Eunice Kennedy Schriver National Institute of Child Health and Human Development [F32 HD062124]
- Burroughs Wellcome Fund
The genome sequence for Schistosoma mansoni has been determined, allowing the complete protein complement to be predicted. However, few functional genomics techniques have been developed for use in S. mansoni, limiting the usefulness of the sequence data. Here we describe a whole mount in situ hybridization (WISH) method that can be used to identify the tissue-specific expression of transcripts in S. mansoni. Using this protocol we determine the tissue-specific expression of tetraspanin 2, a female-enriched tetraspanin, phenol oxidase, the secretory Cu/Zn superoxide dismutase, and an Argonaute family member. The localization of these transcripts by WISH correlates with prior studies performed using immunohistochemistry and/or in situ hybridization on tissue sections. WISH can be adapted to screen multiple transcripts, thus identifying novel targets for drugs or vaccines. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据