期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 174, 期 1, 页码 62-65出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2010.05.010
关键词
Giardia lamblia; Transcriptome; Gene expression; Life cycle; SAGE
资金
- NIH [A142488, GM61896, DK35108, A1051687, A151089]
- Swedish Natural Science Research Council
- Swedish Medical Research Council
- Karolinska Institutet
- Ellison Medical Foundation
- W.M. Keck Foundation
- G. Unger Vetlesen Foundation
We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of G. lamblia. (C) 2010 Elsevier B.V. All rights reserved.
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