期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 166, 期 2, 页码 183-185出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2009.03.001
关键词
Trypanosoma brucei; Protein kinase; Genetic validation; RNA interference; Drug target
资金
- Wellcome Trust [071463]
Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery. (C) 2009 Elsevier B.V. All rights reserved.
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