期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 168, 期 1, 页码 95-101出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2009.07.004
关键词
Cryptosporidium parvum; Human ileocecal epithelial cells; HCT-8; Protein Activated Receptor-2
资金
- National Institutes of Allergy and Infectious Diseases [R01AI055347]
- Agnes Varis Graduate Fellowship
The genus Cryptosporidium includes several species of intestinal protozoan parasites which multiply in intestinal epithelial cells. The impact of this infection on the transcriptome of cultured host cells was investigated using DNA microarray hybridizations. The expression of 14 genes found to be consistently up- or down-regulated in infected cell monolayers was validated with RT PCR. Using immunofluorescence we examined the expression of Protease Activated Receptor-2, which is encoded by one of the up-regulated genes. In infected cells this receptor localized to the host cell membrane which covers the intracellular trophozoites and meronts. This observation indicates that the composition of the host cell membrane is affected by the developing trophozoite, a phenomenon which has not been described previously. (C) 2009 Elsevier B.V. All rights reserved.
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