期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 157, 期 2, 页码 228-232出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2007.10.005
关键词
Toxoplasma gondii; bradyzoite-specific genes; suppression subtractive hybridization; TPR-domain; ankyrin
By using suppression subtractive hybridization we identified five so far uncharacterized stage specific genes in Toxoplasma gondii, which are induced during tachyzoite-to-bradyzoite differentiation. The mRNA level of a putative zinc-finger protein was increased 23-fold in bradyzoites, while the remaining four genes displayed induction levels >100-fold. Two of these genes predict proteins with domains for protein-protein interactions. One protein (ANK1) contains both, a TPR-domain and an ankyrin motif, which consists of seven repeats. ANK1 was shown by epitope tagging experiments to be localized in the cytosol. In a fraction of parasites, the myc-tagged fusion protein was additionally localized in the nucleus, which is in agreement with the presence of a bipartite nuclear targeting sequence in ANK1. The identification of bradyzoite-specific proteins with TPR-and ankyrin-domains supports the concept that during stage conversion a variety of proteins which are involved in protein-protein interactions are induced, thereby assisting the rebuilding of the proteome. (c) 2007 Elsevier B.V. All rights reserved.
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