期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 12, 期 5, 页码 1272-1280出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M112.023168
关键词
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资金
- Leukamie Lymphom Liga e.V. Dusseldorf
- Forschungskommission of the Heinrich-Heine-University, Dusseldorf, Germany
Bleeding complications are a significant clinical problem in patients with myelodysplastic syndromes even at sufficient platelet counts (>50,000/mu l). However, the underlying pathology of this hemorrhagic diathesis is still unknown. Here, we analyzed the platelet proteome of patients with myelodysplastic syndromes by quantitative two-dimensional difference gel electrophoresis followed by mass spectrometric protein identification. Proteins identified with lower concentrations, such as Talin-1, Vinculin, Myosin-9, Filmain-A, and Actin play critical roles in integrin alpha(IIb)beta(3) signaling and thus platelet aggregation. Despite normal agonist receptor expression, calcium flux, and granule release upon activation, the activation capacity of integrin alpha(IIb)beta(3) was diminished in myelodysplastic syndrome platelets. Forster resonance energy transfer analysis showed a reduced co-localization of Talin-1 to the integrin's beta(3)-subunit, which is required for receptor activation and fibrinogen binding. In addition, platelet spreading on immobilized fibrinogen was incomplete, and platelet aggregation assays confirmed a general defect in integrin-dependent platelet aggregation in patients with myelodysplastic syndromes. Our data provide novel aspects on the molecular pathology of impaired platelet function in myelodysplastic syndromes and suggest a mechanism of defective integrin alpha(IIb)beta(3) signaling that may contribute to the hemorrhagic diathesis observed in these patients.
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