期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 11, 期 11, 页码 1416-1429出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M112.019588
关键词
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资金
- Plan Nacional de I + D + i [SAF2008/00461, SAF2011/25681]
- Instituto de Salud Carlos III [RD08/0075]
- Fundacion Ramon Areces
The association of ERAP1 with ankylosing spondylitis (AS) 1 among HLA-B27-positive individuals suggests that ERAP1 polymorphism may affect pathogenesis by altering peptide-dependent features of the HLA-B27 molecule. Comparisons of HLA-B*27:04-bound peptidomes from cells expressing different natural variants of ERAP1 revealed significant differences in the size, length, and amount of many ligands, as well as in HLA-B27 stability. Peptide analyses suggested that the mechanism of ERAP1/HLA-B27 interaction is a variant-dependent alteration in the balance between epitope generation and destruction determined by the susceptibility of N-terminal flanking and P1 residues to trimming. ERAP1 polymorphism associated with AS susceptibility ensured efficient peptide trimming and high HLA-B27 stability. Protective polymorphism resulted in diminished ERAP1 activity, less efficient trimming, suboptimal HLA-B27 peptidomes, and decreased molecular stability. This study demonstrates that natural ERAP1 polymorphism affects HLA-B27 antigen presentation and stability in vivo and proposes a mechanism for the interaction between these molecules in AS. Molecular & Cellular Proteomics 11: 10.1074/mcp.M112.019588, 1416-1429, 2012.
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