期刊
AQUACULTURE
卷 448, 期 -, 页码 105-111出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2015.05.040
关键词
Litopenaeus vannamei; Proline; POX; Immune response; Environment stress
资金
- fund of China Agriculture Research Systerm-47 (CARS-47)
An 8-week feeding trial was conducted to evaluate the effect of proline on anti-oxidative capacity, immune response and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei. Six practical low fishmeal diets (15% fishmeal) were formulated to contain graded levels (2.02, 2.14, 2.27, 2.38, 2.49, 2.6%) of L-proline. Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.41 g) and the shrimps were fed 4 times a day to apparent satiation. Growth performance was not affected by proline levels (P > 0.05). But higher levels of proline increased hydroxyproline content in the whole body (P = 0.004). And survival after 60 h acute NH3 stress increased with the increasing dietary proline levels (P = 0.015). High levels of proline also increased the proline oxidase (POX), nitric oxide (NO), total antioxidant capacity (T-AOC) and phenoloxidase (PO) activities in hemolymph or hepatopancreas after the 60 h NH3 stress compared to basic group which had lowest proline level. Broken-line analysis showed that the optimal dietary proline requirement of L. vannamei was 2.29%, 2.32% and 2.34% based on PO, POX and 60 h survival after NH3 stress. These results clearly indicated that 2.29-2.34% of proline in the low fish meal diet could improve anti-oxidative capacity, immune response, NH3 stress tolerance of L. vannamei, and proline may be a conditionally essential AA for L. vannamei. Statement of relevance: This study indicates that proline may be a conditional amino acid for white shrimp under the environment stress, especially when fed with low fish meal diet. The results will contribute to the low fish meal diet study of the white shrimp. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据