期刊
MITOCHONDRION
卷 12, 期 2, 页码 213-219出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2011.09.001
关键词
Bcl-2; Mcl-1; Bim; Astrocyte; miR-181; Mitochondria
资金
- NIH [NS053898, GM49831]
Mitochondria are central to the execution of apoptosis, and the Bcl-2 protein family of pro- and anti-apoptotic proteins interacts with mitochondria to regulate apoptosis. Using bioinformatics we predicted that miR-181, a microRNA expressed in brain, could target the 3'UTRs of Bcl-2 family members Bcl-2-L11/Bim, Mcl-1, and Bcl-2. Using the luciferase reporter assay we confirmed these targets. We used mimic and inhibitor to alter miR-181a levels in primary astrocyte cultures and found miR-181a reduction was associated with increased Bcl-2 and Mcl-1 protein levels. Decreased miR-181a levels reduced glucose deprivation induced apoptosis, mitochondrial dysfunction, and loss of mitochondrial membrane potential in astrocytes. (C) 2011 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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