期刊
MITOCHONDRION
卷 11, 期 4, 页码 630-634出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2011.04.002
关键词
mtDNA replication; Replication stall; Recombination; Repair; Twinkle
资金
- Academy of Finland
- EMBO Long Term Fellowship [ALTF 1125-2009]
- Sigrid Juselius Foundation
- Tampere University Hospital [9J119, 9K126, 9L097]
- Netherlands Organization for Scientific Research (NWO)
Pathological mitochondrial DNA (mtDNA) rearrangements have been proposed to result from repair of double-strand breaks caused by blockage of mitochondrial DNA (mtDNA) replication. As mtDNA deletions are seen only in post-mitotic tissues, it has been suggested that they are selected out in actively dividing cells. By electron microscopy we observed rearranged mtDNA molecules in cultured human cells expressing a catalytically impaired helicase. As these molecules were undetectable by PCR, we propose that deleted mtDNA molecules in cultured cells are fragile and sensitive to heating. Further consequences of mtDNA replication stalling are discussed. (C) 2011 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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