期刊
MITOCHONDRION
卷 9, 期 4, 页码 242-246出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2009.02.006
关键词
mtDNA depletion syndrome; Mitochondrial DNA; Real-time PCR; DNA isolation and purification methods
资金
- Fondo de Investigacion Sanitaria [PI06/0848, PI07/0347]
Mitochondrial DNA (mtDNA) content is important for understanding many cellular processes. Several pre-analytical factors, from sample collection to DNA extraction can affect measurement of mtDNA copy number. In the present study, whole blood samples yielded a higher mtDNA copy number than buffy coat samples. mtDNA content is affected by the cell separation method used and the time between blood withdrawal and cell separation. Thus, reference values must be established with the same type of sample. As to the DNA isolation and purification method, the manual phenol method can give randomly false high values. The QIAamp DNA Mini Kit provided the most highly reproducible mtDNA/nDNA yield. (C) 2009 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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