期刊
SCIENCE
卷 349, 期 6252, 页码 1120-1124出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa9046
关键词
-
资金
- NIH [GM074815]
- UK Biotechnology and Biological Sciences Research Council [BB/F012004/1]
- Leverhulme Trust [RPG-245]
- DOE Office of Biological and Environmental Research
- MINOS at National Center for Biotechnology Information, NIH [R01-GM105404]
- BBSRC [BB/F012004/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F012004/1] Funding Source: researchfish
Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据