期刊
SCIENCE
卷 349, 期 6254, 页码 1347-1350出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa4938
关键词
-
资金
- National Natural Science Foundation of China [91230116, 31170697, 31370736, 31000333]
- National Basic Research Program of China [2010CB912403]
- Program for New Century Excellent Talents [NCET-13-0787]
- Hunan Provincial NSF [13JJ1017]
Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D-3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据