Effects of dietary supplementation with beta-glucan and synbiotics on immune gene expression and immune parameters under ammonia stress in Pacific white shrimp

The goal of this study was to investigate the effects of dietary supplementation with beta-glucan and synbiotics (i.e., microencapsulated Bacillus subtilis and Pediococcus acidilactici) on immune gene expression and immune parameters of Pacific white shrimp (Litopenaeus vannamei) as well as modulation of immune parameters when shrimp were exposed to ammonia stress. Shrimp were fed one of four treatment diets [basal diet (C), beta-glucan-containing diet (beta-glu), beta-glucan plus B. subtilis-containing diet (beta-glu + Bs), and beta-glucan plus P. acidilactici-containing diet (beta-glu + Pa)] for 90 days. The microencapsulated bacteria colonized in the proximal and central parts of the shrimp intestine. The beta-glu diet increased expression of lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP), and the beta-glu + Bs diet induced expression of the serine protease and prophenoloxidase genes. In addition, beta-glu + Pa increased the LGBP transcription. However, the mRNA level of peroxinectin did not differ significantly among the experimental groups. Shrimp fed the beta-glu and beta-glu + Pa diets had higher superoxide dismutase activity (SOD) compared to shrimp fed the basal diet. In addition, the beta-glu + Bs and beta-glu + Pa diets increased phenoloxidase activity (PO). However, there were no significant differences in total hemocyte count (THC) and lysozyme activity among the treatment diets. Ammonia stress led to increased THC and decreased SOD activity, but it seemed to have no adverse effects on PO and lysozyme activity. Thus, both beta-glucan and synbiotic supplementation had beneficial effects on several immune parameters, and the effect of dietary synbiotics was superior to that of beta-glucan only for PO activity. (C) 2014 Elsevier B.V. All rights reserved.