期刊
SCIENCE
卷 348, 期 6240, 页码 1268-1270出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa2850
关键词
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资金
- National Institutes of Health [AI113643]
- National Science Foundation Graduate Research Fellowship [DGE-1519168]
- National Natural Science Foundation [81420108024]
- Fralin Life Science Institute
- Virginia Agriculture Experimental Station
Sex determination in the mosquito Aedes aegypti is governed by a dominant male-determining factor (M factor) located within a Y chromosome-like region called the M locus. Here, we show that an M-locus gene, Nix, functions as an M factor in A. aegypti. Nix exhibits persistent M linkage and early embryonic expression, two characteristics required of an M factor. Nix knockout with clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 resulted in largely feminized genetic males and the production of female isoforms of two key regulators of sexual differentiation: doublesex and fruitless. Ectopic expression of Nix resulted in genetic females with nearly complete male genitalia. Thus, Nix is both required and sufficient to initiate male development. This study provides a foundation for mosquito control strategies that convert female mosquitoes into harmless males.
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