期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 73, 期 11, 页码 1067-1071出版社
WILEY
DOI: 10.1002/jemt.20836
关键词
bone marrow trephine; glycol methacrylate; immunohistochemistry; fluorescence in situ hybridization; polymerase chain reaction; sequencing
资金
- Nanjing Health Bureau [YKK06086]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, Bureau of Nanjing Personnel, P.R.C. [2005179]
- EPSCoR
- Office Of The Director [GRANTS:13762384, 1003970] Funding Source: National Science Foundation
Molecular analyses such as fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) are demanded to improve diagnostic accuracy in addition to immunohistopathology of bone marrow (BM) trephine specimens. Conventional BM embedding method needs decalcification, and its procedure may impair tissue morphology and DNA quality. Here, we report an undecalcified method by which glycol methacrylate resin is polymerized at low temperature (4 degrees C). Using this method, BM enzyme activity and antigenic determinants are well preserved, and moreover, DNA extracted from plastic embedding sections is suitable for PCR amplification and sequencing, FISH analysis can be well done because of the DNA integrity of BM sections. If working with BM trephine specimen, our protocol offers the possibility to combine superior morphology with modern molecular analysis. Microsc. Res. Tech. 73: 1067-1071, 2010. (C) 2010 Wiley-Liss, Inc.
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