期刊
MICROSCOPY AND MICROANALYSIS
卷 16, 期 4, 页码 456-461出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1431927610093645
关键词
lipid nanoparticles; USPIO; staining process; TEM
Lipid-containing nanostructures, in the form of solid lipid nanoparticles or iron oxide nanoparticles (NPs) coated with a lipid shell, were used as case studies for assessing and optimizing staining for transmission electron microscopy structural and compositional characterization. These systems are of paramount importance as drug delivery systems or as bio-compatible contrast agents. In particular, we have treated the systems with a negative (phospshotungstic acid) or with a positive (osmium tetroxide) staining agent. For iron-oxide NPs coated with the lipid shell, negative staining was more efficient with respect to the positive one. Nevertheless, in particular cases the combination of the two staining procedures provided more complete morphological and compositional characterization of the particles.
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