期刊
MICROPOROUS AND MESOPOROUS MATERIALS
卷 199, 期 -, 页码 57-65出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micromeso.2014.08.003
关键词
Zeolite; Composite material; Laccase; Dye degradation; MALDI-ToF-MS
类别
资金
- Scientific and Technological Research Council of Turkey (TUBITAK) [113Z427]
Poly(2-chloroethyl acrylate), p(CEA), as a functional polymer was grafted via surface initiated atom transfer radical polymerization (SI-ATRP) on zeolite particles. The p(CEA) grafted zeolite particles were aminated, and a ligninolytic enzyme laccase from Trametes versicolor was covalently immobilized on the composite particles via glutaraldehyde coupling. The amount of immobilized laccase on the zeolite-g-p(CEA) particles was determined as 17.7 mg g(-1) particles. The degradation of a model dye (i.e., Reactive Red 120; RR-120) with the free and immobilized laccases was evaluated in batch and continuous systems. The covalently immobilized laccase showed a high decolorization efficiency toward RR-120 in aqueous solution compared to the free counterpart. The resulting dye degradation products were analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI ToF-MS). (C) 2014 Elsevier Inc. All rights reserved.
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