期刊
MICROPOROUS AND MESOPOROUS MATERIALS
卷 155, 期 -, 页码 24-33出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micromeso.2012.01.014
关键词
Periodic mesoporous organosilicas (PMOs); Immobilized enzyme; HRP; Activity enhancement of enzyme; Synthesis
类别
资金
- National NSFC [20871067, 20873059, 21173117]
- MSTC [2008AA062327]
- National Basic Research Program of China [2007CB613301]
- NMOE [XY1110284038]
- Analysis Centre of Nanjing University
A new class of periodic mesoporous organosilicas (PMOs), which contains various hydrophilic groups of -OH, -O- and -S-, is successfully synthesized with novel bridged organosilica precursor under the acid-catalyzed and inorganic-salt-assisted conditions. These hybrid materials possess the ordered mesostructure with uniform pore size distributions, thick framework walls, thermal stability and specific functional groups; and they are carefully characterized by XRD, N-2 adsorption, TGA, FT-IR, TEM and NMR spectroscopies. Moreover, horseradish peroxidase (HRP) is immobilized on these new PMO composites for the first time, and their enzyme activity in the oxidation of 1,3,5-trimethylbenzene (TMB) is elevated in the range of 25-59% compared with that of free HRP in solution. Again, the HRP immobilized samples can be reused for many cycles, resulting from the interfacial activation of the enzyme when it is attached to the surface of hybrid hosts where is filled with organic groups and hydrophobic parts. (C) 2012 Elsevier Inc. All rights reserved.
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