期刊
MICROPOROUS AND MESOPOROUS MATERIALS
卷 122, 期 1-3, 页码 201-207出版社
ELSEVIER
DOI: 10.1016/j.micromeso.2009.03.002
关键词
Mesostructured silica; Drug delivery; Cell-penetrating peptide; Sigmoidal release; Sustained cellular uptake
类别
资金
- Japan Science and Technology Agency (JST)
- Swedish Governmental Agency for Innovation Systems (VINNO-VA)/Swedish Foundation for Strategic Research (SSF)
- National Natural Science Foundation of China [20425102, 20890121, 20821140537]
- NSF [2009CB930403]
- Swedish Research Council
A drug delivery system using mesostructured silica as a reservoir has been developed for the storage and controlled release of a drug with a cell-penetrating peptide (CPP) as a vector. We use fluorescein isothiocyanate (FITC) as the drug model and octaarginine (R8) as a vector to endow the drug with cell-penetrating property. The mesostructured silica reservoir system was prepared by using a one-pot liquid-crystal templating method, which is suitable for the encapsulation of intact FITC-R8 conjugates and sustained release of drugs without hampering their properties. The hydrophobic poly(propyl oxide) (PPO) shell of the pore-filling Pluronic F127 and the electrostatic interaction between R8 and siloxide ions on the pore walls act as the diffusion-limiting factors of the FITC-R8 conjugate. A sigmoidal in vitro release of FITC-R8 from mesostructured silica into phosphate buffered saline (PBS, pH 7.4) was observed and the typical release duration was 5 days at 37 degrees C. Release from the reservoir yielded significant elongation in duration of the FITC signals in DUI 45 cells by confocal microscopic analysis, compared with a single administration of FITC-R8. (C) 2009 Elsevier Inc. All rights reserved.
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