期刊
MICRON
卷 42, 期 2, 页码 117-131出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2010.06.003
关键词
Cryo-negative staining; Negative staining; Biological assemblies; Macromolecular structure; TEM
类别
资金
- NIH/NCRR/RCMI [G12-RR03060]
In this review we cover the technical background to negative staining of biomolecules and viruses, and then expand upon the different possibilities and limitations. Topics range from conventional air-dry negative staining of samples adsorbed to carbon support films, the variant termed the negative staining-carbon film technique and negative staining of samples spread across the holes of holey-carbon support films, to a consideration of dynamic/time-dependent negative staining. For each of these approaches examples of attainable data are given. The cryo-negative staining technique for the specimen preparation of frozen-hydrated/vitrified samples is also presented. A detailed protocol to successfully achieve cryo-negative staining with ammonium molybdate is given, as well as examples of data, which support the claim that cryo-negative staining provides a useful approach for the high-resolution study of macromolecular and viral structure. (C) 2009 Elsevier Ltd. All rights reserved.
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