期刊
MICRON
卷 42, 期 4, 页码 348-352出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2010.03.006
关键词
Sub-diffraction limit microscopy; Localisation microscopy; Spectral Precision Distance Microscopy (SPDM); Fluorescence
类别
资金
- University Heidelberg
- Deutsche Forschungsgemeinschaft [SPP1128]
- European Union
- Federal Ministry of Education and Research (BMBF)
Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200 nm laterally and 600 nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the lambda/10 range (similar to 50 nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions. (C) 2010 Elsevier Ltd. All rights reserved.
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