期刊
MICRON
卷 40, 期 3, 页码 285-301出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2008.10.002
关键词
Amyloid; Anomalous colours; Birefringence; Congo red; Dichroism; Polarising microscopy
类别
Amyloid stained by Congo red has striking optical properties that generally have been poorly described and inadequately explained, although they can be understood from principles of physical optics. Molecules of Congo red are orientated on amyloid fibrils, and so the dye becomes dichroic and birefringent. The birefringence varies with wavelength in accordance with a fundamental property of all light-transmitting materials called anomalous dispersion of the refractive index around an absorption peak, The combination of this and absorption of light, with modification by any additional birefringence in the optical system, explains the various Colours that can be seen in Congo red-stained amyloid between crossed polariser and analyser, and also when the polariser and analyser are progressively uncrossed. These are called anomalous colours. (C) 2008 Elsevier Ltd. All rights reserved.
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