期刊
MICRON
卷 40, 期 8, 页码 911-917出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2009.05.007
关键词
Osmium tetroxide; mu CT; X-ray; Contrast; Respiratory organ
类别
资金
- DFG [Scho 442/10-1]
Remarkable results obtained while searching for suitable contrasting agents for tomographical imaging are presented. Book lungs, the respiratory organs of scorpions (Scorpiones, Arachnida), served as challenging objects in terms of fragility and preparatory requirements, as well in terms of size differences between the entire organ and its functional subunits (the lamellae). The problem of tissue contrast was investigated using various metals, but most successfully addressed using osmium. Gaseous osmium tetroxide exposure with subsequent ethanol treatment yielded the best results. A constant and thorough staining of the book lungs was achieved, easing book-lung visualization using 3D-reconstructions from mu CT scans. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据