期刊
MICRON
卷 39, 期 2, 页码 77-87出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2006.11.003
关键词
field emission scanning electron microscopy; cell surface; cell cytoskeleton; parasite-host cell interaction
类别
Field emission scanning electron microscopy (FE-SEM) provides a range of strategies for investigating the structural organization of biological systems, varying from isolated macromolecules to tissue organization and whole organisms. This review covers some of the results so far obtained using FE-SEM observation and various protocols of sample fixation to analyze the structural organization of parasitic protozoa and their interaction with host cells. The employment of FE-SEM can be broadened through the use of gold-labeled molecules or tracers, gradual extraction by detergents, and cleavage techniques. These analyses provide significant contributions to the characterization of these organisms concerning ultrastructure, cytoskeleton, motility and intracellular behavior. (C) 2006 Elsevier Ltd. All rights reserved.
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