期刊
MICROFLUIDICS AND NANOFLUIDICS
卷 15, 期 1, 页码 117-126出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10404-012-1133-1
关键词
Lab-on-a-chip; Droplet; Mixing; Valving; ESI-MS detection
资金
- William R. Wiley Environmental Molecular Sciences Laboratory (EMSL)
- NIH National Center for Research Resources [RR018522]
- US DOE's Office of Biological and Environmental Research
- DOE [DE-AC05-76RLO 1830]
We present an integrated droplet-on-demand microfluidic platform for dispensing, mixing, incubating, extracting and analyzing by mass spectrometry pico- to nanoliter-sized droplets. All of the functional components are successfully integrated for the first time into a monolithic microdevice. Droplet generation is accomplished using computer-controlled pneumatic valves. Controlled actuation of valves for different aqueous streams enables accurate dosing and rapid mixing of reagents within droplets in either the droplet generation area or in a region of widening channel cross-section. Following incubation, which takes place as droplets travel in the oil stream, the droplet contents are extracted to an aqueous channel for subsequent ionization at an integrated nanoelectrospray emitter. Using the integrated platform, rapid enzymatic digestions of a model protein were carried out in droplets and detected online by nanoelectrospray ionization mass spectrometry.
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