期刊
MICROFLUIDICS AND NANOFLUIDICS
卷 9, 期 2-3, 页码 411-426出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10404-009-0558-7
关键词
Dielectrophoresis; Microfluidics; Yeast; Sorting
This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of similar to 80% at low medium conductivities and similar to 85% at high medium conductivities.
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