期刊
MICROELECTRONIC ENGINEERING
卷 88, 期 8, 页码 1852-1855出版社
ELSEVIER
DOI: 10.1016/j.mee.2010.12.060
关键词
Microfluidics; Janus particle; Dielectrophoresis; Colloid; Covalent coupling; Adsorption
Janus particles have proven to be original particles to sense protein-cell interactions or to organize cell on a substrate. This work presents a covalent coupling strategy to graft fibronectin on a specific side of Au/PS or Au/SiO2 1 mu m Janus particles. By studying several buffers that could limit adsorption of protein on plain particles of PS, SiO2 and Au, fibronectin is covalently grafted on those particles. We demonstrate two ways of grafting proteins on one side of the Janus particles, either on the Au side functionalized with thiols or on the PS/SiO2 side activated with carboxylate groups. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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