期刊
MICROELECTRONIC ENGINEERING
卷 88, 期 10, 页码 3094-3100出版社
ELSEVIER
DOI: 10.1016/j.mee.2011.06.003
关键词
Electrochemical impedance spectroscopy (EIS); Cell plasma membrane; Single cell measurement; Ultra-microelectrode (UME)
资金
- Dankook university
The electrochemical characteristics of the plasma membranes of single cells and organelles were investigated. Silicon fabrication technology was used to produce a metal ultra-microelectrode (UME). Furthermore, the UME was characterized in a cell medium using electrochemical impedance spectroscopy (EIS). A single rat fibroblast cell, or chloroplast purified from Peperomia metallica leaves, was immobilized by a micropipette after which the UME was inserted into its cytosolic space through cell membrane using a piezo actuator. An in vivo EIS measurement between the UME and the counter electrode outside of a single cell was taken. The measurements were analyzed using equivalent circuits in order to estimate the membrane impedance of a single cell. (C) 2011 Elsevier B.V. All rights reserved.
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