期刊
MICROCIRCULATION
卷 15, 期 2, 页码 123-135出版社
WILEY
DOI: 10.1080/10739680701447415
关键词
microelectrode; NO; resistance; blood flow
资金
- NHLBI NIH HHS [HL-20605, R01 HL020605-28, R01 HL020605] Funding Source: Medline
Objective: Arginine used for nitric oxide formation can be from intracellular stores or transported into cells. The study evaluated the rapidity., and primary site of NO and vascular resistance responses to arginine at near physiological concentrations (100-400 mu M). Methods: Arginine was applied to a single arteriole through a micropipette to determine the fastest possible responses. For vascular blood flow and [NO] responses., arginine was added to the bathing media. Results: Dilation of single arterioles to arginine began in 10-15 seconds and application over the entire vasculature increased [NO] in similar to 60-90 seconds, and flow increased within 120-300 seconds. Resting periarteriolar [NO] for arterioles was 493.6 +/- 30.5 nM and increased to 696.1 +/- 68.2 and 820.1 +/- 110.5 nM at 200 and 400 mu M L-arginine. The blood How increased 50% at 400-1200 mu M L-arginine. The reduced arterial resistance during topical arginine was significantly greater than microvascular resistance at 100 and 200 mu M arginine. All responses were blocked by L-NAME. Conclusions: This study demonstrated arterial resistance responses are as or more responsive to arginine induced NO formation as arterioles at near Physiological concentrations of arginine. The vascular NO and resistance responses occurred rapidly at L-arginine concentrations at and below 400 mu M., which predict arginine transport processes were involved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据