期刊
MICROCHIMICA ACTA
卷 182, 期 3-4, 页码 571-578出版社
SPRINGER WIEN
DOI: 10.1007/s00604-014-1360-0
关键词
Aflatoxin B1; Aptamer; Quantum dot; Graphene oxide; Fluorescence
资金
- National Key Basic Research Program of China (973 Program) [2013CB127804]
- National Natural Science Foundation of China [21205097]
- Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies
- Southwest University [SWU111071]
- Chongqing International Collaboration Base for Science and Technology (Southwest University)
- Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing, China
- Fundamental Research Funds for the Central Universities [XDJK2012C005]
- Chongqing Natural Science Foundation [cstc2012jjA1137]
Aflatoxin B1 (AFB1), a secondary fungal metabolite of Aspergillus flavus, was employed as a model mycotoxin to establish an aptamer based assay that exploits the quenching of the fluorescence of CdTe quantum dots (Q-dots) by graphene oxide (GO). A thiolated aptamer specific for AFB1 was linked to the surface of Q-dots via ligand exchange. The fluorescence of the aptamer modified-Q-dots is strongly quenched by GO. If, however, AFB1 is added, fluorescence is restored depending on the quantity of AFB1 added. The system was evaluated both in phosphate buffer solution and in peanut oil. If performed in an aqueous system, the assay possesses good selectivity, a wide dynamic range (from 3.2 nM to 320 mu M) and a low limit of detection (1.0 nM). If performed in peanut oil solution, the dynamic range is from 1.6 nM to 160 mu M, and the limit of detection is 1.4 nM. In our perception, this is a simple, sensitive and selective method for the determination of AFB1 that also may be extended to the analysis of other mycotoxins.
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