期刊
MICROCHIMICA ACTA
卷 182, 期 3-4, 页码 479-486出版社
SPRINGER WIEN
DOI: 10.1007/s00604-014-1344-0
关键词
Ag nanoparticle; m-aminophenol; Resin microsphere; H2O2; Glucose; Biosensor
资金
- Chinese 973 Project [2012CB933302]
- National Natural Science Foundation of China [21175022]
- Ministry of Science & Technology of China [2012AA022703]
- Scientific Research Foundation of Graduate School of Southeast University [YBJJ1410]
- Fundamental Research Funds for the Central Universities [2242014Y10054]
- Graduate Students' Scientific Research Innovation Project of Jiangsu Province Ordinary University [CXZZ13_0124]
An enzymatic glucose biosensor was developed that is making use of microspheres consisting of an m-aminophenol based resin decorated with silver nanoparticles (AgNP/MAPR). It was applied to the detection of H2O2 and, if loaded with glucose oxidase (GOx), to the determination of glucose. The AgNPs were thermally immobilized on the MAPR microspheres. The AgNP/MAPR microspheres were deposited on a glassy carbon electrode (GCE) by surface casting. The resultant electrode exhibits remarkable catalytic performance in terms of H2O2 reduction. The sensor gives an amperometric response to H2O2 within < 3 s and has a linear response in the 0.1 mM to 0.18 M concentration range (r = 0.997). The detection limit is 2.7 mu M at an SNR of 3. The glucose biosensor has a linear response in the 2 to 12 mM range and a detection limit of similar to 0.1 mM. The biosensor was applied to the determination of glucose in human blood serum.
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