期刊
MICROCHIMICA ACTA
卷 181, 期 15-16, 页码 1917-1929出版社
SPRINGER WIEN
DOI: 10.1007/s00604-014-1294-6
关键词
Quercetin; AuNPs; Arginine; Histidine; Lysine; UV-visible spectrometry
资金
- DST, India under DST-Inspire Fellowship Ph.D. Programme
We report on the use of quercetin-functionalized gold nanoparticles (QC-AuNPs) as a colorimetric probe for the amino acids arginine (Arg), histidine (His) and lysine (Lys). The method is based on the aggregation of the QC-AuNPs that is caused by these amino acids and leads to a visually detectable color change from red to blue. The absorption maxima shift from 525 nm to 702, 693, and 745 nm, respectively. Aggregations are confirmed by dynamic light scattering (DLS) and transmission electron microscopic techniques (TEM). The effects of the QC concentration, temperature and reaction time for the preparation of QC-Au NPs were tested. Other amino acids do not interfere. Under the optimal conditions, linear relationships exist between the absorption ratios at 702/525 nm (for Arg), 693/525 nm (for His), and 745/525 nm (for Lys) over the concentrations ranges from 2.5-1,250 mu M (Arg) and 1-1,000 mu M (His and Lys), respectively. The respective limits of detection are 0.04, 0.03, and 0.02 mu M. The method provides a useful tool for the rapid visual and instrumental determination of the three amino acids.
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