期刊
MICROCHIMICA ACTA
卷 180, 期 5-6, 页码 397-403出版社
SPRINGER WIEN
DOI: 10.1007/s00604-013-0945-3
关键词
Surface plasmon resonance; Biosensor; MicroRNA; Singal amplification; Streptavidin
资金
- National Natural Science Foundation of China [21075141]
- Special Fund Project for Key Laboratory of Chongqing
We are presenting a method for sensitive and specific detection of microRNA (miRNA) using surface plasmon resonance. A thiolated capture DNA probe with a short complete complementary sequence was immobilized on the gold surface of the sensor to recognize the part sequence of target miRNA, and then an oligonucleotide probe linked to streptavidin was employed to bind the another section of the target. The use of the streptavidin-oligonucleotide complex caused a similar to 5-fold increase in signal, improved the detection sensitivity by a factor of similar to 24, and lowered the detection limit to 1.7 fmol of miR-122. This specificity allowed a single mismatch in the target miRNA to be discriminated. The whole assay takes 30 min, and the surface of the sensor can be regenerated at least 30 times without loss in performance. The method was successfully applied to the determination of miRNA spiked into human total RNA samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据