期刊
MICROCHIMICA ACTA
卷 181, 期 3-4, 页码 381-387出版社
SPRINGER WIEN
DOI: 10.1007/s00604-013-1121-5
关键词
Titanium dioxide; Nanotube arrays; Glucose oxidase; Biosensor; Cross-linking
资金
- National Natural Science Foundation of China [21373047, 20871029]
- Research Fund for the Doctoral Program of Higher Education of China [200802861071]
- Program for New Century Excellent Talents in University of the State Ministry of Education [NCET-08-0119]
- Science & Technology Program of Suzhou City [SYG201017, ZXG2012026, SYN201208]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University [2011E17]
A glucose biosensor has been fabricated by immobilizing glucose oxidase (GOx) on unhybridized titanium dioxide nanotube arrays using an optimized cross-linking technique. The TiO2 nanotube arrays were synthesized directly on a titanium substrate by anodic oxidation. The structure and morphology of electrode material were characterized by X-ray diffraction and scanning electron microscopy. The electrochemical performances of the glucose biosensor were conducted by cyclic voltammetry and chronoamperometry measurements. It gives a linear response to glucose in the 0.05 to 0.65 mM concentration range, with a correlation coefficient of 0.9981, a sensitivity of 199.6 mu A mM(-1) cm(-2), and a detection limit as low as 3.8 A mu M. This glucose biosensor exhibited high selectivity for glucose determination in the presence of ascorbic acid, sucrose and other common interfering substances. This glucose biosensor also performed good reproducibility and long-time storage stability. This optimized cross-linking technique could open a new avenue for other enzyme biosensors fabrication.
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