期刊
MICROCHIMICA ACTA
卷 180, 期 15-16, 页码 1487-1493出版社
SPRINGER WIEN
DOI: 10.1007/s00604-013-1077-5
关键词
TiO2 nanorod film; Hydrogen peroxide biosensor; Horseradish peroxidase; Electrocatalysis
资金
- National Basic Research Program of China (973 Program) [2012CB933600]
- National Natural Science Foundation of China [51072178, 51272228, 81071258]
Titanium dioxide nanorods (TNR) were grown on a titanium electrode by a hydrothermal route and further employed as a supporting matrix for the immobilization of nafion-coated horseradish peroxidase (HRP). The strong electrostatic interaction between HRP and TNR favors the adsorption of HRP and facilitates direct electron transfer on the electrode. The electrocatalytic activity towards hydrogen peroxide (H2O2) was investigated via cyclic voltammetry and amperometry. The biosensor exhibits fast response, a high sensitivity (416.9 mu A center dot mM(-1)), a wide linear response range (2.5 nM to 0.46 mM), a detection limit as low as 12 nM, and a small apparent Michaelis-Menten constant (33.6 mu M). The results indicate that this method is a promising technique for enzyme immobilization and for the fabrication of electrochemical biosensors.
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