期刊
MICROCHIMICA ACTA
卷 181, 期 1-2, 页码 205-212出版社
SPRINGER WIEN
DOI: 10.1007/s00604-013-1100-x
关键词
Electrochemical sensor; Aptamer; Lipid bilayer membrane; Adenosine triphosphate
资金
- National Science Foundation of People's Republic of China [21275100]
- Shanghai Leading Academic Discipline Project [S30406]
- Key Laboratory of Resource Chemistry of Ministry of Education
We have developed an aptasensor for adenosine triphosphate (ATP) based on an electrode-supported lipid bilayer membrane. The assay is based on a conformational change that is induced after binding the target which modulates the electron transfer rate in the conductive path. The method is highly sensitive, stable, and repeatable. The detection limit for ATP is 50 nM, and the dynamic range extends to 3.2 mu M, which covers the concentration range of ATP in cell lysates (from 0.1 to 1 mu M). The method also holds promise in that it may be transferred to submicro- or nano-scale electrodes so to enable intracellular monitoring of ATP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据