期刊
MICROCHIMICA ACTA
卷 180, 期 15-16, 页码 1517-1522出版社
SPRINGER WIEN
DOI: 10.1007/s00604-013-1068-6
关键词
Bovine serum albumin; In situ synthesis; Platinum nanoparticles; Colorimetric detection
资金
- National Natural Science Foundation of China [21075078, 21105056]
- Natural Science Foundation of Shandong province, China [ZR2010BM005, ZR2011BQ001]
Platinum nanoparticles (Pt-NPs) with sizes in the range from 10 to 30 nm were synthesized using protein-directed one-pot reduction. The model globular protein bovine serum albumin (BSA) was exploited as the template, and the resulting BSA/Pt-NPs were studied by transmission electron microscopy, energy dispersive X-ray spectroscopy, and resonance Rayleigh scattering spectroscopy. The modified nanoparticles display a peroxidase-like activity that was exploited in a rapid method for the colorimetric determination of hydrogen peroxide which can be detected in the 50 mu M to 3 mM concentration range. The limit of detection is 7.9 mu M, and the lowest concentration that can be visually detected is 200 mu M.
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