期刊
MICROCHIMICA ACTA
卷 178, 期 1-2, 页码 147-152出版社
SPRINGER WIEN
DOI: 10.1007/s00604-012-0828-z
关键词
Ratiometric fluorescence; Nanoparticles; Oxygen sensing; Intracellular
资金
- National Natural Science Foundation of China [61078069, 10979009]
- National Science Fund for Distinguished Young Scholars [61125505]
- Fundamental Research Funds for the Central Universities [2010JBZ006]
A facile reprecipitation-encapsulation method is used for the preparation of ratiometric fluorescent nanoparticles (NPs) for sensing intracellular oxygen. The surface of the NPs is modified in-situ with poly-L-lysine, which renders good biocompatibility and enables easy internalization into living cells. The sensor NPs contain a red fluorescent probe whose fluorescence is sensitive to oxygen with a quenching response of 77 % on going from nitrogen saturation to oxygen saturation, and a reference dye giving a green signal that acts as an oxygen-independent reference. The ratio of the two emissions serves as the analytical information and is sensitive to dissolved oxygen in the 0-43 ppm concentration range. When incorporated into cells, the ratio of the signals increases by 400 % on going from oxygen-saturated to oxygen-free environment.
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