期刊
MICROCHIMICA ACTA
卷 177, 期 1-2, 页码 89-94出版社
SPRINGER WIEN
DOI: 10.1007/s00604-011-0744-7
关键词
Gold nanoparticles; Guanine-rich oligonucleotide; Pb2+; Colorimetric assay
资金
- National Natural Science Foundation of China [60671014, 20775012]
We have developed a colorimetric method for the determination of Pb(II) ions. It is based on the use of gold nanoparticles and a guanine-rich synthetic oligonucleotide. On addition of Pb(II), the color of the solution turns from red to blue. The ratio of the UV-vis absorption at 630 nm and 525 nm is proportional to the concentration of Pb(II) ions in the range from 10 to 100 nM, and the detection limit is 20 nM. Other metal ions do not interfere if present in up to a 10-fold molar excess. The method was successfully applied to the detection of Pb(II) in lake water and urine. The recovery in case of spiked samples is 92%. The results show that this method is sensitive, simple and fast.
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