期刊
MICROCHIMICA ACTA
卷 178, 期 1-2, 页码 37-43出版社
SPRINGER WIEN
DOI: 10.1007/s00604-012-0808-3
关键词
DNA damage; Glucose oxidase; Co(bpy)(3)(3+); Hydroxyl radical; Electrochemistry
资金
- National Natural Science Foundation of China [20875023]
- Natural Science Fund for Creative Research Groups of Hubei Province of China [2011CDA111]
We report on a biosensor for the electrochemical detection of the damage of DNA and of antioxidant protecting DNA. The biosensor was constructed by co-immobilization of DNA and glucose oxidase (GOx) on a glassy carbon electrode. Under aerobic conditions, GOx catalyzes the oxidation of glucose, and the hydrogen peroxide produced reacts with ferrous ions in a Fenton-type reaction to generate hydroxy radical. This was validated by UV-vis spectroscopy. The hydroxy radical can cause serious oxidative damage to DNA, and this can be detected by square wave voltammetry of the electroactive indicator Co(bpy) (3) (3+) . The effects of pH value, incubation time, and the concentration of glucose and ferrous ion were optimized. The effects of the antioxidants ascorbic acid and aloe emodin on DNA damage were also investigated within the concentration range from 0.05 to 200 mu M. This work provides an in-vitro model system to mimic the processes in oxidative DNA damage by a simple electrochemical approach.
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