期刊
MICROCHIMICA ACTA
卷 174, 期 3-4, 页码 303-309出版社
SPRINGER WIEN
DOI: 10.1007/s00604-011-0616-1
关键词
Tyrosinase; Multiwalled carbon nanotubes; Electrochemical biosensors; Phenols
资金
- National Natural Science Foundation of China [20247002]
- Beijing Natural Science Foundation [8102009]
- Beijing Municipal Education Commission Scientific Technological Project Foundation [KZ201110005006]
- Beijing Municipal institution of higher learning academic innovating group [PHR 201007105]
We describe a biosensor for phenolic compounds that is based on a glassy carbon electrode modified with tyrosinase immobilized on multiwalled carbon nanotubes (MWNTs). The MWNTs possess excellent inherent electrical conductivity which enhances the electron transfer rate and results in good electrochemical catalytic activity towards the reduction of benzoquinone produced by enzymatic reaction. The biosensor was characterized by cyclic voltammetry, and the experimental conditions were optimized. The cathodic current is linearly related to the concentration of the phenols between 0.4 mu M and 10 mu M, and the detection limit is 0.2 mu M. The method was applied to the determination of phenol in water samples.
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