期刊
MICROCHIMICA ACTA
卷 171, 期 3-4, 页码 399-405出版社
SPRINGER WIEN
DOI: 10.1007/s00604-010-0441-y
关键词
Gold; Silicon dioxide; Nanoparticle; Electrodeposition; NADH; Ethanol
资金
- National Nature Science Foundation of China [20705041]
- Natural Science Foundation of Hubei Province [2007ABA127]
- Nature Science Foundation of the South-Central University for Nationalities [XTZ09005]
- South-Central University for Nationalities [ZZZ10002]
A new electrode was developed by one-step potentiostatic electrodeposition (at -2.0 V for 20 s) of Au/SiO2 nanoparticles on a glassy carbon electrode. The resulting electrode (nano-Au/SiO2/GCE) was characterized by scanning electronic microscopy, X-ray photoelectron spectroscopy and electrochemical techniques. The electrochemical behavior of dihydronicotinamide adenine dinucleotide (NADH) at the nano-Au/SiO2/GCE were thoroughly investigated. Compared to the unmodified electrode, the overpotential decreased by about 300 mV, and the current response significantly increased. These changes indicated that the modified electrode showed excellent catalytic activity in the oxidation of NADH. A linear relationship was obtained in the NADH concentration range from 1.0x10(-6) to 1.0x10(-4) molL(-1). In addition, amperometric sensing of ethanol at the nano-Au/SiO2/GCE in combination with alcohol dehydrogenase and nicotinamide adenine dinucleotide was successfully demonstrated. A wide linear response was also found for ethanol in the range from 5.0x10(-5) to 1.0x10(-3) molL(-1) and 1.0x10(-3) to 1.0x10(-2) mol L-1, respectively. The method was successfully applied to determine ethanol in beer and biological samples.
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