期刊
MICROCHIMICA ACTA
卷 168, 期 3-4, 页码 341-345出版社
SPRINGER WIEN
DOI: 10.1007/s00604-010-0289-1
关键词
Quantum dots; Calf thymus DNA; Photoinduced electron transfer; Ru(bpy)(3)(2+)
资金
- National Natural Science Foundation of China [20675034, 20975042]
- Program for academic pacesetter of Wuhan [200851430484]
- Genetically Modified major projects [2009ZX08012-015B]
- Nature Science foundation key project from Hubei Province of China [2008CDA080]
A method has been developed for the rapid determination of calf thymus (ct) DNA that is based on the photoinduced electron transfer (PET) that occurs between CdTe quantum dots and the ruthenium(II)tris-bipyridyl complex. The latter quenches the photoluminescence (PL) of the quantum dots through PET. The Stern-Volmer quenching constant is 2,500 L mol(-1). The intensity of the PL the system is recovered in the presence of ct DNA, and relative recovered PL intensity is linearly proportional to the concentration of ct-DNA. The dynamic range is from 17 A mu M to 1.5 mM of DNA, and the detection limit (at S/N = 3) is 5.7 A mu M. The relative standard deviation (at 0.5 mM of ct-DNA) is 4.1% (n = 11). A possible reaction mechanism is discussed.
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