A new luminol chemiluminescence reaction using a tetravalent nickel-periodate complex as the oxidant

We report on a new chemiluminescence (CL) reaction that occurs between luminol and di-periodatonickelate in alkaline medium. The possible mechanism of this system is discussed based on the kinetics of the reaction, its CL and UV spectra. The system exhibits a sensitivity that is higher than any other system based on luminol. It was successfully applied to the determination of isoniazid in serum.