期刊
MICROCHEMICAL JOURNAL
卷 112, 期 -, 页码 25-33出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2013.09.012
关键词
Multivariate curve resolution; Doxorubicin; Human plasma; Second-order calibration
资金
- Universidad Nacional del Litoral [11-11, 11-7]
- CONICET (Consejo Nacional de Investigaciones Cientificas y Tecnicas) [455]
- ANPCyT (Agencia Nacional de Promocion Cientifica y Tecnologica)
this paper, the development of a pH-gradient flow-injection method with diode-array spectrophotometric detection combined with extended multivariate curve resolution-alternating least-squares (MCR-ALS) to quantitate doxorubicin in the presence of rifampicin in plasma samples is presented. Quantitation of doxorubicin was based on acid-base and spectral differences between its species and those for the interference. Overlapped profiles among the analyte and the interference were mathematically solved by data modeling with the well-known extended MCR-ALS algorithm. The method, which involves a simple sample pretreatment consisting in the addition of trichloroacetic acid followed by centrifugation, was applied to resolve validation mixtures composed of human plasma spiked with doxorubicin. Acceptable analytical figures of merit were obtained, i.e. recoveries from 96 to 107%, coefficients of variation between 0.2% and 7.8%, and limits of detection and quantification of 0.77 and 232 mu g L-1, respectively. (C) 2013 Elsevier B.V. All rights reserved.
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