期刊
MICROCHEMICAL JOURNAL
卷 99, 期 2, 页码 503-507出版社
ELSEVIER
DOI: 10.1016/j.microc.2011.07.001
关键词
Potassium ion; G-quadruplex; Berberine; Fluorescence sensor
资金
- Chinese Ministry of Education [109150]
- Fundamental Research Funds for the Central Universities [GK2009011004]
In this work, a novel potassium ion (K+) sensor is presented using berberine-G-quadruplex complex as a fluorescent probe. This sensor is based on the K(+)that can induce the G-rich DNA to form G-quadruplex conformation. The G-quadruplex can bind berberine to form berberine-G-quadruplex complex, resulting in remarkable enhancement of fluorescence emission of the berberine-G-quadruplex system. In the presence of 800 mM sodium ion (Na+), the fluorescence of the berberine-G-quadruplex complex increased linearly with increasing K+ concentration in the range of 0.005-1.0 mM. The turn-on fluorescent assay is simple, inexpensive, and highly sensitive. We observed that Na+ in 10,000-fold molar excess does not interfere. The molecular mechanisms which produce enhanced fluorescence of berberine were discussed. (C) 2011 Elsevier B.V. All rights reserved.
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