4.2 Article

Self-association of the Shigella flexneri IcsA autotransporter protein

期刊

MICROBIOLOGY-SGM
卷 158, 期 -, 页码 1874-1883

出版社

MICROBIOLOGY SOC
DOI: 10.1099/mic.0.056465-0

关键词

-

资金

  1. National Health and Medical Research Council (NHMRC) of Australia
  2. University of Adelaide
  3. Australian Postgraduate Award (NHMRC)

向作者/读者索取更多资源

The IcsA autotransporter protein is a major virulence factor of the human intracellular pathogen Shigella flexneri. IcsA is distributed at the poles in the outer membrane (OM) of S. flexneri and interacts with components of the host actin-polymerization machinery to facilitate intracellular actin-based motility and subsequent cell-to-cell spreading of the bacterium. We sought to characterize the biochemical properties of IcsA in the bacterial OM. Chemical cross-linking data suggested that IcsA exists in a complex in the OM. Furthermore, reciprocal co-immunoprecipitation of differentially epitope-tagged IcsA proteins indicated that IcsA is able to self-associate. The identification of IcsA linker-insertion mutants that were negatively dominant provided genetic evidence of IcsA-IcsA interactions. From these results, we propose a model whereby IcsA self-association facilitates efficient actin-based motility.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.2
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据