期刊
MICROBIOLOGY-SGM
卷 158, 期 -, 页码 166-175出版社
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.052670-0
关键词
-
类别
资金
- Hi-Tech Research and Development Program of China [2011AA090704]
- National Natural Science Foundation of China [30870047, 81071804]
- Specialized Research Fund for the Doctoral Program of Higher Education [BA2009YY009]
- Program of Shandong for Taishan Scholars
- Natural Science Foundation of Shandong Province [JQ200910, 2008BS02019]
- Independent Innovation Foundation of Shandong University [2009TS079, 2011DX002]
Antibiosis is one of the widespread strategies used by Trichoderma spp. against plant fungal pathogens, the mechanism of which, however, remains poorly understood. Peptaibols are a large family of antimicrobial peptides produced by Trichoderma spp. Our previous study showed that trichokonins, a type of peptaibol from Trichoderma pseudokoningii SMF2, exhibited antibiotic activities against plant fungal pathogens. In this study, we first demonstrated that trichokonin VI (TK VI) induced extensive apoptotic programmed cell death in plant fungal pathogens. For a deeper insight into the apoptotic mechanism involved in the action of TK VI, Fusarium oxysporum was used as a model. Cells of F. oxysporum treated with TK VI showed apoptotic hallmarks, such as exposure of phosphatidylserine, the appearance of reactive oxygen species and fragmentation of nuclear DNA. Moreover, TK VI-treated cells exhibited an accumulation of cytoplasmic vacuoles with loss of the mitochondrial transmembrane potential, and this process was independent of metacaspases. Therefore, TK VI induces metacaspase-independent apoptotic cell death in F oxysporum. This represents what is believed to be the first report to reveal the antibiotic mechanism of peptaibols against plant fungal pathogens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据