期刊
MICROBIOLOGY AND IMMUNOLOGY
卷 52, 期 12, 页码 625-630出版社
WILEY
DOI: 10.1111/j.1348-0421.2008.00079.x
关键词
particle formation; SARS-CoV; VLP
资金
- Ministry of Education, Science, Sports, and Culture of Japan [18780225]
- National Project on Protein Structural and Functional Analyses
- Grants-in-Aid for Scientific Research [18780225] Funding Source: KAKEN
SARS-CoV has four major structural proteins: the N, S, M, and E proteins. To investigate the mechanism of SARS-CoV assembly, we cloned the genes encoding these four proteins into the eukaryotic expression vector pCAGGS and transfected them into 293T cells. When all four expression vectors were co-transfected VLP formed, as confirmed using electron microscopy. Using a rabbit polyclonal antibody specific to the N protein, N-protein-containing particles similar in size to the VLP were also observed by immunoelectron microscopy, indicating that the VLP contained the N protein. Co-immunoprecipitation analyses demonstrated an interaction between the N and M proteins, suggesting that N protein binds directly to M protein to be incorporated into VLP.
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